Immediately overlay each IPG strip with mineral oil to prevent evaporation and precipitation of urea during rehydration. See Table 2. Without the IPG strip retainers, electrolysis gasses may lift IPG strips off of the electrodes, interrupting electrical contact. Place IPG strip retainers after the focusing tray has been placed into the instrument to avoid movement of the strip retainers. Rehydration in the focusing tray with in-gel sample application can be programmed as a part of the IEF run or be performed separately.
To program rehydration as part of the run:. Select or program the protocol s for the lanes containing IPG strips see Section 3. Program the global rehydration conditions in the Run Settings screen see Section 3. If electrode wicks are used, include a pause to insert electrode wicks when the rehydration step is completed. Start the run see Section 2.
For rehydration not programmed as part of the run, leave the tray on the Peltier platform or on a level bench overnight 12—18 hr for complete rehydration.
A self-diagnostic program runs for approximately 10 sec. On the user interface, a message reads Self Test in Progress. If a component fails, the diagnostic program stops, and an error message appears see Chapter 7, Troubleshooting.
Once the cell is powered on, the Main screen appears Figure 2. Start the run by selecting one of the options described in Figure 2. Create a new protocol with up to 10 steps in the Edit Protocol screen Chapter 4. Welcome to ManualMachine. We have sent a verification link to to complete your registration. Log In Sign Up. Forgot password? Enter your email address and check your inbox. Please check your email for further instructions.
Enter a new password. Legal Notices No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad Laboratories. All rights reserved. Preface Safety and Regulatory Compliance This instrument has been certified to meet all applicable requirements of the EN electrical equipment for measurement, control, and laboratory use standard and the class A standards for Electromagnetic Emissions, intended for laboratory equipment applications.
Instrument Safety Warnings This instrument should not be modified or altered in any way. This individual power control for each lane allows use of IPG strips with different pH gradients and sample types, concentrations, and conductivities in a single run. Component Quantity Description.
Electrode Assemblies The electrode assemblies Figure 1. IEF workflow overview. Square and round orientation pins in the electrode assemblies ensure correct positioning of the cathode and anode in the focusing tray Release clips secure the electrode assemblies onto the focusing tray Green tabs on the release clips help push the electrode assemblies into place. To place the electrode assemblies in the i12 focusing tray: 1.
Placement of an electrode assembly onto the i12 focusing tray. Platform positioning guide Tray positioning stop Positioning the tray Tray positioning Metal grounding strip guide Tray positioning Negative pin stop Platform positioning guide Instrument platform Fig. Connecting the electrodes. Chapter 2 Basic Operation 2. Rehydration in the focusing tray with in-gel sample application can be programmed as part of the IEF run See Appendix B, Sample Loading Methods and Running Configurations, for guidelines for selecting the sample loading method and IPG strip configuration.
Chapter 2 Basic Operation 3. IPG strip positioning stop C Place electrode assemblies in the focusing tray. Press down on the green tabs to snap the assemblies into place. Placement of an IPG strip gel-side up into the i12 focusing tray. B Recommended Place wet electrode wicks in the channels of the focusing tray.
A Place both electrode assemblies in the focusing tray. C Place IPG strips gel-side down in the channels of the focusing tray. IPG strip positioning stop Fig. Stores 9 methods, each with up to 9 steps. Dimensions W x L x H. Our technicians decontaminate and clean equipment upon arrival at our facility and upon your purchase to create a neutral environment for your labs use.
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Learn More - opens in a new window or tab. Report item - opens in a new window or tab. Step Procedure Description Connect electrophoresis Insert power leads into one of the output terminals located on cells.
Page 16 Press Setup once to return to the previous screen or twice to exit setup mode. The changes. NOTE: The template method is automatically saved with the new parameters. Page Manual Mode Operation The limit parameters Limits appear on the right in smaller text on two lines, and are set to their respective maximum values.
While paused, you can edit the run see Section 3. Page Method Mode Operation Press the down arrow key to accept the method name.
Page 23 Method screen. If you need to add more steps, proceed to Step 6; otherwise, skip to Step 7 to save the method. Page The Run Stopped screen appears. Complete the step definition procedure in Step 5 page After you are finished creating the necessary steps for the method, proceed to Step 7 to save the method.
The example the follows shows the new method name as Another1. View the run information.
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